High fat diet RNA seq hypothalamus

By | May 11, 2021

high fat diet RNA seq hypothalamus

Hypothalamic inflammation: a double-edged sword to nutritional diseases. Additional file 2: Table S2. A List of lncRNAs significantly changed in high fat diet fed mouse cortex. D HFD dams have significantly higher average daily energy intake MJ during the three week diet and pregnancy but not lactation. Shao, Y. The data in Supplementary Figure S3 were used for the quantitation. Heat maps were generated with the pheatmap package in R using rlog values. The dams remained on the respective diets throughout mating, pregnancy and until the end of study at PN10 Fig 1. Under light restraint tail blood was drawn and glycemia measured before 0 minutes and after injection at 15, 30, 60, and minutes.

A range of Open source clustering software. The primers used for gene validation are listed in Additional file 1 : Table S1. Changes in brain activity after a diet-induced obesity. Bioinformatics 30, — JS and Y-KK wrote the manuscript. Lipoic acid attenuates high-fat-diet-induced oxidative stress and B-cell-related immune depression. Quattrocki E, Friston K. Inhibition of glial cell proinflammatory activities by peroxisome proliferator-activated receptor gamma agonist confers partial protection during antimyelin oligodendrocyte glycoprotein demyelination in vitro. Hypothesis testing was conducted using statistical analysis GraphPad Prism 6.

Interestingly, altered expression hypoghalamus collagens have been identified in other diet in high postnatal overfeeding model [ 36 ]. Diet and Hypothalamus further demonstrated that offspring of seq fat diet fed rats demonstrated a disrupted response to glucose-sensitive genes in the hypothalamus in RNA to insulin [ 20 ]. Transcription RNA Sox8 and Sox10 perform non-equivalent roles during oligodendrocyte development despite functional redundancy. Tissue samples included the whole hypothalamus and heart, liver, spleen, lung, kidney, stomach, duodenum, skeletal muscle and blood. Glucose tolerance seq GTT was performed as described previously No study high reported the expression of fat in the brain from high fat diet fed mouse either. Tarsa, Fat. The maximum number allowed for mismatch mapping was 2. Mol Endocrinol.

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You seq high hypothalamus fat diet RNA ready helpTo date, no study characterises the genome-wide transcriptional changes that occur in response to exposure to high fat diet during this critical window. We explored the effects of maternal high fat diet consumption on hypothalamic gene expression in Sprague Dawley rat offspring at postnatal day RNA-sequencing enabled discovery of differentially expressed genes between offspring of dams fed a high fat diet and offspring of control diet fed dams. Male high fat diet offspring showed significant changes in collagen genes Col1a1 and Col3a1 and significant upregulation of two genes involved in regulation of dopamine availability in the brain, tyrosine hydroxylase Th and dopamine reuptake transporter Slc6a3 also known as Dat1.
Seq diet high fat hypothalamus RNA can consultHigh fat diet can lead to metabolic diseases such as obesity and diabetes known to be chronic inflammatory diseases with high prevalence worldwide. Recent studies have reported cognitive dysfunction in obese patients is caused by a high fat diet. Therefore, the objective of this study was to obtain profiles of diverse RNAs and determine their patterns of alteration in high fat fed brain cortex compared to normal brain cortex. Our study provides valuable expression profiles and potential function of both coding and non-coding RNAs in high fat diet fed brain cortex.
All hypothalamus seq fat diet high RNA remarkable words recommendSoybean oil consumption has increased greatly in the past half-century and is linked to obesity and diabetes. To test the hypothesis that soybean oil diet alters hypothalamic gene expression in conjunction with metabolic phenotype, we performed RNA sequencing analysis using male mice fed isocaloric, high-fat diets based on conventional soybean oil high in linoleic acid, LA, a genetically modified, low-LA soybean oil Plenish, and coconut oil high in saturated fat, containing no LA. The 2 soybean oil diets had similar but nonidentical effects on the hypothalamic transcriptome, whereas the coconut oil diet had a negligible effect compared to a low-fat control diet. Oxytocin immunoreactivity in the supraoptic and paraventricular nuclei of the hypothalamus was reduced, whereas plasma oxytocin and hypothalamic Oxt were increased.
Share fat seq RNA high hypothalamus diet come forum andMetrics details. The effects of short-term high fat diets on physiology are elusive and the molecular changes following fat overconsumption remain largely unknown. Global gene expression of ten important tissues brain, heart, liver, spleen, lung, kidney, stomach, duodenum, skeletal muscle and blood was analyzed using RNA Sequencing. Principle component analysis revealed that differential gene expression patterns existed in the ten tissues.
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